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hpi2 dylight 405  (Novus Biologicals)


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    Novus Biologicals hpi2 dylight 405
    Hpi2 Dylight 405, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hpi2+dylight+405/HPi2+Antibody+(HIC1-2B4%2E2B)+%5BDyLight+405%5D/pmc12256296-33-22-24
    Average 93 stars, based on 1 article reviews
    hpi2 dylight 405 - by Bioz Stars, 2026-09
    93/100 stars

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    FACS:

    Article Title: Isolation of Live Human δ Cells for Genetic and Functional Analysis.
    Article Snippet: Afterwards, cells were washed with cold PBS twice and then labeled with the LIVE/DEAD Fixable Near-IR stain (Life Technologies) using manufacture’s recommended condition (1ul dye per 10E7 cells for 30-minute incubation at 4 o C). .. Next, cells were washed with cold FACS buffer (2% v/v fetal bovine serum, 10mM EGTA in PBS) prior to blocking with normal mouse IgG (Jackson ImmunoResearch, 1:20 dilution in FACS buffer) for 10 minutes at 4 o C . To label cell-type specific surface markers we used the following conjugated primary antibodies mix for 30-minutes at 4 o C : islet cells by HPi2-DyLight 405 (Novus Biological) in 1:100 dilution, β and δ cells by NTPDase3-DyLight 650 (Jean Sévigny PhD, Université Laval) in 1:75 dilution, and α cells by CD26-Biotin as well as Brilliant Violet 605 Streptavidin (BioLegend). .. Labeled cells were sorted on a special order five-laser FACS Aria II (BD Biosciences) using a 100-μm nozzle, with compensation controls and doublet removal.

    Blocking Assay:

    Article Title: Isolation of Live Human δ Cells for Genetic and Functional Analysis.
    Article Snippet: Afterwards, cells were washed with cold PBS twice and then labeled with the LIVE/DEAD Fixable Near-IR stain (Life Technologies) using manufacture’s recommended condition (1ul dye per 10E7 cells for 30-minute incubation at 4 o C). .. Next, cells were washed with cold FACS buffer (2% v/v fetal bovine serum, 10mM EGTA in PBS) prior to blocking with normal mouse IgG (Jackson ImmunoResearch, 1:20 dilution in FACS buffer) for 10 minutes at 4 o C . To label cell-type specific surface markers we used the following conjugated primary antibodies mix for 30-minutes at 4 o C : islet cells by HPi2-DyLight 405 (Novus Biological) in 1:100 dilution, β and δ cells by NTPDase3-DyLight 650 (Jean Sévigny PhD, Université Laval) in 1:75 dilution, and α cells by CD26-Biotin as well as Brilliant Violet 605 Streptavidin (BioLegend). .. Labeled cells were sorted on a special order five-laser FACS Aria II (BD Biosciences) using a 100-μm nozzle, with compensation controls and doublet removal.



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    hpi2 dylight 405  (Novus Biologicals)
    93
    Novus Biologicals hpi2 dylight 405
    Hpi2 Dylight 405, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hpi2+dylight+405/HPi2+Antibody+(HIC1-2B4%2E2B)+%5BDyLight+405%5D/pmc12256296-33-22-24
    Average 93 stars, based on 1 article reviews
    hpi2 dylight 405 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

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